MIST CHAMBERS

Mist Chambers are another useful tool for the healthy production of healthy vegetation or agriculture. Mist chambers are common to every greenhouse or to the plant lovers but are they all able to utilize it properly for the purpose they have in their mind.

Mist Chambers are helpful in controlling the loss of Plants moisture by periodic wetting of the foliage of rooted cuttings. Mist chambers are a good source of maintaining ambient humidity and temperature rooting of cuttings of Eucalyptus, Casuarine, Bamboos and all medicinal plants species. In this environment in mist chambers, all tissue-cultured plants can get the higher productivity. You can numerous varieties of Mist chambers according to the purpose of your vegetation. Mist chambers also come with a quality for cloning of forest species.

Saveer Biotech Limited also offers a good range of mist range with best possible conditions for growth and development of plants. We are dedicated our worth time to offer our clients a complete mist chamber that is widely used in the cultivation of different types of vegetation and organs and seeds. With the conditions of growth and development of Plants we provide high quality mist chambers that help in reducing the loss of plant moisture and helpful for rooting leafy cuttings. These are used for growing plants with their roots such as forest plantation, herbs, medicinal plants and trees etc. along with germ free nutrient solutions.

Saveer Biotech Limited is mist chambers manufacturer in Delhi, India. We design mist chambers that can easily be adjusted in terms of temperature, humidity, light, benching systems and many more.

TISSUE CULTURE LAB AND ITS EQUIPMENTS

Tissue Culture is a process of generating many plants through technique of placing cell or tissue in a desired environment of Plants. They demands proper supply of artificial light, humidity and controlled temperature conditions. PTC has a main quality that from any part of mother plant, a hub of plants can be generated. To produce such plants we need a laboratory equipped with different rooms or sections to perform the process of producing plants in the Laboratory.

MEDIA PREPARATION ROOM- Media preparation room needs a spacious environment for the storage of chemicals, glass culture and equipments to manufacture the media. It holds all the essential materials that complete the role of Plant Tissue Culture Laboratory and below is the list of media preparation room’s equipments.

EQUIPEMENTS AND BASIC MATERIAL IN PLANT TISSUE CULTURAL LAB:

• Hot plate / magnetic stirrer or stove
• Glassware (measuring cups, Erlenmeyer) or stainless steel to heat up and dissolve the media Equipment with pressure steam sterilization (autoclave)
• pH meter Scales (analytical and bench top loading)
• Glass measuring gradual
• Culture bottle with lid
• Dispenser
• Tools dissection (spatula, scalpel (tweezers), forceps, scissors)
• Refrigerator
• Distilling units or water deionizer
• Oven and Microwave
• Microscope
• Pipette measuring
• Shaker
• Laminar air flow
• Disinfectant
• Chemicals needed to manufacture the media (Appendix) etc.

In the manufacture of media, everything is done step by step with the proper use of equipments at the demand of situation.

WASH ROOM- Wash room is a place which needs to be spacious so that it can carry all necessary equipments to maintain the plants regularly. It should have a sink or wash basin and a desk made of materials resistant to acids and alkalis to prevent the unwanted results at the time of using acid on plants for their treatment.  A storage cabinet or equipment rack to place or store them for long as well as space for oven, washing machine and dryers is important.

SPACE TRANSFER- In transfer area, there are very dry and clean conditions that need to maintained to perform successful operations on an open laboratory bench. Within the transfer area it requires proper flow of air, light and electricity, compressed gas and vacuum. The most useful environment is a small dust free room facilitated with ultra-violet light and a positive pressure ventilation unit.

CULTURE ROOM– All types of plant tissue culture should be developed under conditions of proper temperature, humidity, light and air flow etc. These factors influence the growth of plants during culture or can indirectly affect their response in subsequent generations.

PLANT TISSUE CULTURE- Medium of Growth

We have seen many beautiful plants and flowers that we have wished to have them in hundreds and thousands whether to beautify the garden or for sell purpose. Tissue culture is the process for the growth of animal cells but for the plants cells we use more defined term called Plant Tissue culture.

Plant Tissue culture helps in protecting the agriculture growth, which are resistant to pests, viral or bacterial disease. It helps in providing food to countries that are combating starvation due to famine, drought and bad economical conditions etc.  This also helps in stocking the food by the process of micro propagation to produce the food in large number and to utilize it properly. Plant Tissue culture is also named as Micro propagation, which was introduced by a German researcher, Haberlandt in 1902. Later in 1934, Gautheret discovered effective results on in-vitro culture of plants. It has been proved to be an experimental and artificial method with the use of plant element (e.g. bud, leaves, single and tissue etc.) to produce the plant in multi numbers.

Tissue culture needs proper maintenance and observation, which can provide proper growth to multi plants in equality. The cells must be contained under defined environment conditions. They are various applications of tissue culturing, depending on the tissue used including specialized functions and replacing damaged cells.

Seed germination also have their applications in the following areas:

• Forestry research
• Seed testing
• Biological products
• Plant tissue culture

Application of tissue culture

• Micro propagation– It is a rapid vegetative multiplication of valuable plant material, which is used mainly for agriculture, horticulture, and forestry.
• Production of disease-free plants– When the apex of shoot is used for multiplication by tissue culture, we get disease free plants because the shoot apical meristem, a group of dividing cells at the tip of a stem or root, is free from pathogens.
• Plant breeding– Tissue culture has also been successfully used in plant breeding program. Therefore, it is now used for general purposes also.
• Production of disease and pest resistant plants– Plants grown from tissue culture usually pass trough callus phase and show many variations. These show some agronomic characteristics like tolerance to pests, diseases, etc.

Advantages:

• It is very helpful in the genetically modified organism studies.
• To produce many copies of the same plants, which may be used to produce plants with better flowers, odors, fruits or any other properties of the plants are beneficial to the human beings.
• To produce plants anytime we want appropriate climate. Moreover, if seeds are not available, it is possible to produce a plant with its method too.
• If there is a plant with partially infected tissue, it is possible to produce a new plant without infection.
• Useful solution for the prevention of starvation in third world countries since the process is highly efficient, by using only one plant.
• The equipments are cheaper than compared to the animal cell culture.

Disadvantages:

• If large scale production is being thought, the costs of the equipments are very expensive.
• The procedure is very variable and it depends on the type of the species so sometimes it needs trial-and-error type of experiments if there is not any review about that species.
• The procedure needs special attention and diligently done observation.
• There may be error in the identity of the organisms after culture.
• Infection may continue through generations easily if possible precautions are not taken
• Decrease genetic variability.

Saveer Biotech Ltd. is well known Greenhouse Suppliers in India, also manufacture commercial greenhouse farming in India and a range of other technologically advanced products which support in organic agriculture.